DNA

Part:BBa_K2100005:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)


pENTR eYFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 222
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 222
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 222
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 222
    Illegal AgeI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a gene derived from a jellyfish's genome.

References